The coupled effect of light and temperature on dormancy release and germination of Pinus koraiensis seeds

Abstract Elucidating the regulatory mechanisms of environmental factors on seed dormancy and germination will provide guidance for tree regeneration. Toward understanding coupled effect light temperature release Pinus koraiensis seeds, we set up three conditions (L200: 200 μmol m −2 s −1 , L20: 20 L0: 0 μ ) four storage temperatures [T-5: − 5 °C (50 days), T5: days) + T25: °C(50 25 T15: 15 days)] using imbibed then quantified phytohormones gibberellic acid (GA 3 abscisic (ABA) during stratification. Germination percentage ( G P ), mean time T M value V under 25/15 were determined. Phytohormone levels performances significantly affected by temperature. No consistent trend was found between phytohormone caused levels. Under conditions, ABA concentrations in embryo endosperm decreased as shifted from T-5 to T25 increased T15; GA nearly all temperatures. reached 40–60% without irradiance. In higher at T5 than so are considered optimum germination. At temperatures, (L200, L20) compared with dark (L0). L200 L20, significant negative correlations positive GA/ABA found. Temperature played a more important role primary germination; unnecessary release. Light facilitated The P. seeds controlled decrease or an increase induced variations.